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Scientists Use Gentle To Alter Genes
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For additional validation, we selected histone H3 lysine 27 acetylation because this modification has not been previously shown to be altered in CRC 19Protein immunostaining on western blots confirmed the elevated K27 acetylation at H3 (Determine three ) and immunohistochemical staining of paired CRC and corresponding regular tissue sections revealed nuclear localisation of acetylated H3 protein (Determine four ).
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